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Proteintech kiaa1429 rabbit polyclonal antibody

Comprehensive analysis of the <t>KIAA1429</t> in the TCGA and GEO database (A) The expression of KIAA1429 in the TCGA-KIRCC database (tumor samples n = 523, normal samples n = 100). (B–D) The expression levels of KIAA1429 in ccRCC tissues and adjacent normal tissues (23 pairs of paired samples) in (B) GSE36895 dataset, (C) GSE53757 dataset (72 pairs of paired samples), and (D) GSE40435 dataset (101 pairs of paired samples). (E) The GEPIA database analyzed the expression of KIAA1429 in patients with ccRCC of different clinical stages. (F and G) The GEPIA database analyzed the effect of KIAA1429 mRNA expression on the OS and DFS of patients with ccRCC. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Kiaa1429 Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kiaa1429+rabbit+polyclonal+antibody/NY-ESO-1+Antibody/pmc13018871-43-0-5
Average 94 stars, based on 6 article reviews

kiaa1429 rabbit polyclonal antibody - by Bioz Stars, 2026-10

94/100 stars

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1) Product Images from "KIAA1429 promotes clear cell renal cell carcinoma progression by regulating MYC mRNA stability"

Article Title: KIAA1429 promotes clear cell renal cell carcinoma progression by regulating MYC mRNA stability

Journal: iScience

doi: 10.1016/j.isci.2026.115198

Comprehensive analysis of the KIAA1429 in the TCGA and GEO database (A) The expression of KIAA1429 in the TCGA-KIRCC database (tumor samples n = 523, normal samples n = 100). (B–D) The expression levels of KIAA1429 in ccRCC tissues and adjacent normal tissues (23 pairs of paired samples) in (B) GSE36895 dataset, (C) GSE53757 dataset (72 pairs of paired samples), and (D) GSE40435 dataset (101 pairs of paired samples). (E) The GEPIA database analyzed the expression of KIAA1429 in patients with ccRCC of different clinical stages. (F and G) The GEPIA database analyzed the effect of KIAA1429 mRNA expression on the OS and DFS of patients with ccRCC. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Figure Legend Snippet: Comprehensive analysis of the KIAA1429 in the TCGA and GEO database (A) The expression of KIAA1429 in the TCGA-KIRCC database (tumor samples n = 523, normal samples n = 100). (B–D) The expression levels of KIAA1429 in ccRCC tissues and adjacent normal tissues (23 pairs of paired samples) in (B) GSE36895 dataset, (C) GSE53757 dataset (72 pairs of paired samples), and (D) GSE40435 dataset (101 pairs of paired samples). (E) The GEPIA database analyzed the expression of KIAA1429 in patients with ccRCC of different clinical stages. (F and G) The GEPIA database analyzed the effect of KIAA1429 mRNA expression on the OS and DFS of patients with ccRCC. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Techniques Used: Expressing, Two Tailed Test

KIAA1429 mRNA and protein expression was significantly upregulated in ccRCC tissues and cell lines and its relationship to clinicopathological parameters (A and B) The protein and mRNA levels of KIAA1429 in ccRCC and normal kidney tissues. (C and D) KIAA1429 mRNA and protein expression in ccRCC cell lines and normal epithelium cell of renal tubule (HK2) cells. (E and F) The expression level of KIAA1429 in ccRCC tissue and surrounding normal renal tissue was detected using IHC. Scale bars, 100 μm. (G) The expression level of KIAA1429 in patients with ccRCC with different clinical stages and Fuhrman’s grade. (H) Effect of KIAA1429 expression on the overall survival of patients with ccRCC. T, tumor; N, normal. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Figure Legend Snippet: KIAA1429 mRNA and protein expression was significantly upregulated in ccRCC tissues and cell lines and its relationship to clinicopathological parameters (A and B) The protein and mRNA levels of KIAA1429 in ccRCC and normal kidney tissues. (C and D) KIAA1429 mRNA and protein expression in ccRCC cell lines and normal epithelium cell of renal tubule (HK2) cells. (E and F) The expression level of KIAA1429 in ccRCC tissue and surrounding normal renal tissue was detected using IHC. Scale bars, 100 μm. (G) The expression level of KIAA1429 in patients with ccRCC with different clinical stages and Fuhrman’s grade. (H) Effect of KIAA1429 expression on the overall survival of patients with ccRCC. T, tumor; N, normal. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Techniques Used: Expressing, Two Tailed Test

KIAA1429 promoted ccRCC cell proliferation in vitro (A) Knockdown efficiencies of KIAA1429 mRNA in ACHN and Caki-1 cells were detected using RT-qPCR; KIAA1429 protein expression decreased significantly in groups of shKIAA1429 in western blot analysis. (B and C) The effect of KIAA1429 knockdown on the proliferation of ccRCC cells was detected using a CCK-8 assay (B) and colony formation assay (C). (D) The effect of KIAA1429 on the cell cycle of ccRCC cells was detected using flow cytometry. Data are presented as the means ± SEM ∗ p < 0.05, ∗∗ p < 0.01 by Student’s two-tailed t test.

Figure Legend Snippet: KIAA1429 promoted ccRCC cell proliferation in vitro (A) Knockdown efficiencies of KIAA1429 mRNA in ACHN and Caki-1 cells were detected using RT-qPCR; KIAA1429 protein expression decreased significantly in groups of shKIAA1429 in western blot analysis. (B and C) The effect of KIAA1429 knockdown on the proliferation of ccRCC cells was detected using a CCK-8 assay (B) and colony formation assay (C). (D) The effect of KIAA1429 on the cell cycle of ccRCC cells was detected using flow cytometry. Data are presented as the means ± SEM ∗ p < 0.05, ∗∗ p < 0.01 by Student’s two-tailed t test.

Techniques Used: In Vitro, Knockdown, Quantitative RT-PCR, Expressing, Western Blot, CCK-8 Assay, Colony Assay, Flow Cytometry, Two Tailed Test

KIAA1429 promoted ccRCC cell migration and invasion in vitro (A and B) The effect of KIAA1429 knockdown on the migration and invasion of Caki-1 and ACHN cells were detected using a cell wound-healing assay (A) and transwell assay (B). (C) Western blot analysis of vimentin, N-cadherin, and E-cadherin protein expression in Caki-1 and ACHN cells with KIAA1429 knockdown. Data are presented as the means ± SEM. ∗ p < 0.05 by Student’s two-tailed t test.

Figure Legend Snippet: KIAA1429 promoted ccRCC cell migration and invasion in vitro (A and B) The effect of KIAA1429 knockdown on the migration and invasion of Caki-1 and ACHN cells were detected using a cell wound-healing assay (A) and transwell assay (B). (C) Western blot analysis of vimentin, N-cadherin, and E-cadherin protein expression in Caki-1 and ACHN cells with KIAA1429 knockdown. Data are presented as the means ± SEM. ∗ p < 0.05 by Student’s two-tailed t test.

Techniques Used: Migration, In Vitro, Knockdown, Wound Healing Assay, Transwell Assay, Western Blot, Expressing, Two Tailed Test

KIAA1429 promoted tumorigenesis of ccRCC cells in vivo (A) Subcutaneous tumor model of ACHN cells with KIAA1429 knockdown. KIAA1429 knockdown led to decreased tumor volume. (B and C) The changes in volume and weight were measured at the indicated weeks after mice were transplanted with the relevant cells. (D) IHC of vimentin, Ki-67, and E-cadherin protein expression in tumors with KIAA1429 knockdown. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Figure Legend Snippet: KIAA1429 promoted tumorigenesis of ccRCC cells in vivo (A) Subcutaneous tumor model of ACHN cells with KIAA1429 knockdown. KIAA1429 knockdown led to decreased tumor volume. (B and C) The changes in volume and weight were measured at the indicated weeks after mice were transplanted with the relevant cells. (D) IHC of vimentin, Ki-67, and E-cadherin protein expression in tumors with KIAA1429 knockdown. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Techniques Used: In Vivo, Knockdown, Expressing, Two Tailed Test

MYC might be a potential target for regulation by KIAA1429 in ccRCC (A) KIAA1429 has a significant effect on most RNA-specific processes, cell cycle, cell death, apoptosis, and intracellular signaling pathways, as revealed by GO and KEGG analyses. (B) Heatmap illustrating the downregulated genes in ACHN cells after KIAA1429 knockdown. (C) The expression level of MYC in ccRCC, obtained from the TCGA database. (D) Correlation analysis between the expression of MYC and KIAA1429. Data are presented as the means ± SEM. ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Figure Legend Snippet: MYC might be a potential target for regulation by KIAA1429 in ccRCC (A) KIAA1429 has a significant effect on most RNA-specific processes, cell cycle, cell death, apoptosis, and intracellular signaling pathways, as revealed by GO and KEGG analyses. (B) Heatmap illustrating the downregulated genes in ACHN cells after KIAA1429 knockdown. (C) The expression level of MYC in ccRCC, obtained from the TCGA database. (D) Correlation analysis between the expression of MYC and KIAA1429. Data are presented as the means ± SEM. ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Techniques Used: Protein-Protein interactions, Knockdown, Expressing, Two Tailed Test

KIAA1429 regulated the expression of MYC mRNA and its stability in an m6A-dependent manner (A and C) KIAA1429 knockdown inhibits the protein and mRNA expression of MYC in ccRCC cells. (B) KIAA1429 knockdown decreased the stability of MYC mRNA. (D) RIP assay showed that KIAA1429 interacts with MYC mRNA in Caki-1 and ACHN cells. (E) KIAA1429 knockdown reduced overall m6A levels in Caki-1 and ACHN cells. (F) KIAA1429 knockdown reduced m6A levels in MYC. (G) m6A methylation sites on MYC were predicted using the SRAMP database. (H) The pmirGLO-MYC-WT or pmirGLO-MYC-MUT luciferase reporter was transfected into KIAA1429-knockdown and control Caki-1 and ACHN cells, and relative luciferase activity was measured. Data are presented as the ratio of firefly to Renilla luciferase activity. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Figure Legend Snippet: KIAA1429 regulated the expression of MYC mRNA and its stability in an m6A-dependent manner (A and C) KIAA1429 knockdown inhibits the protein and mRNA expression of MYC in ccRCC cells. (B) KIAA1429 knockdown decreased the stability of MYC mRNA. (D) RIP assay showed that KIAA1429 interacts with MYC mRNA in Caki-1 and ACHN cells. (E) KIAA1429 knockdown reduced overall m6A levels in Caki-1 and ACHN cells. (F) KIAA1429 knockdown reduced m6A levels in MYC. (G) m6A methylation sites on MYC were predicted using the SRAMP database. (H) The pmirGLO-MYC-WT or pmirGLO-MYC-MUT luciferase reporter was transfected into KIAA1429-knockdown and control Caki-1 and ACHN cells, and relative luciferase activity was measured. Data are presented as the ratio of firefly to Renilla luciferase activity. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Techniques Used: Expressing, Knockdown, Methylation, Luciferase, Transfection, Control, Activity Assay, Two Tailed Test

Expressing:

Article Title: KIAA1429 promotes clear cell renal cell carcinoma progression by regulating MYC mRNA stability
Article Snippet: KIAA1429 Rabbit Polyclonal antibody , Proteintech , Cat24761-1-AP.

Two Tailed Test:

In Vitro:

Knockdown:

Quantitative RT-PCR:

Western Blot:

CCK-8 Assay:

Colony Assay:

Flow Cytometry:

Migration:

Wound Healing Assay:

Transwell Assay:

In Vivo:

Protein-Protein interactions:

Methylation:

Luciferase:

Transfection:

Control:

Activity Assay:

Image Search Results

Journal: iScience

Article Title: KIAA1429 promotes clear cell renal cell carcinoma progression by regulating MYC mRNA stability

doi: 10.1016/j.isci.2026.115198

Figure Lengend Snippet: Comprehensive analysis of the KIAA1429 in the TCGA and GEO database (A) The expression of KIAA1429 in the TCGA-KIRCC database (tumor samples n = 523, normal samples n = 100). (B–D) The expression levels of KIAA1429 in ccRCC tissues and adjacent normal tissues (23 pairs of paired samples) in (B) GSE36895 dataset, (C) GSE53757 dataset (72 pairs of paired samples), and (D) GSE40435 dataset (101 pairs of paired samples). (E) The GEPIA database analyzed the expression of KIAA1429 in patients with ccRCC of different clinical stages. (F and G) The GEPIA database analyzed the effect of KIAA1429 mRNA expression on the OS and DFS of patients with ccRCC. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Article Snippet: KIAA1429 Rabbit Polyclonal antibody , Proteintech , Cat#24761-1-AP.

Techniques: Expressing, Two Tailed Test

Journal: iScience

Article Title: KIAA1429 promotes clear cell renal cell carcinoma progression by regulating MYC mRNA stability

doi: 10.1016/j.isci.2026.115198

Figure Lengend Snippet: KIAA1429 mRNA and protein expression was significantly upregulated in ccRCC tissues and cell lines and its relationship to clinicopathological parameters (A and B) The protein and mRNA levels of KIAA1429 in ccRCC and normal kidney tissues. (C and D) KIAA1429 mRNA and protein expression in ccRCC cell lines and normal epithelium cell of renal tubule (HK2) cells. (E and F) The expression level of KIAA1429 in ccRCC tissue and surrounding normal renal tissue was detected using IHC. Scale bars, 100 μm. (G) The expression level of KIAA1429 in patients with ccRCC with different clinical stages and Fuhrman’s grade. (H) Effect of KIAA1429 expression on the overall survival of patients with ccRCC. T, tumor; N, normal. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Article Snippet: KIAA1429 Rabbit Polyclonal antibody , Proteintech , Cat#24761-1-AP.

Techniques: Expressing, Two Tailed Test

Journal: iScience

Article Title: KIAA1429 promotes clear cell renal cell carcinoma progression by regulating MYC mRNA stability

doi: 10.1016/j.isci.2026.115198

Figure Lengend Snippet: KIAA1429 promoted ccRCC cell proliferation in vitro (A) Knockdown efficiencies of KIAA1429 mRNA in ACHN and Caki-1 cells were detected using RT-qPCR; KIAA1429 protein expression decreased significantly in groups of shKIAA1429 in western blot analysis. (B and C) The effect of KIAA1429 knockdown on the proliferation of ccRCC cells was detected using a CCK-8 assay (B) and colony formation assay (C). (D) The effect of KIAA1429 on the cell cycle of ccRCC cells was detected using flow cytometry. Data are presented as the means ± SEM ∗ p < 0.05, ∗∗ p < 0.01 by Student’s two-tailed t test.

Article Snippet: KIAA1429 Rabbit Polyclonal antibody , Proteintech , Cat#24761-1-AP.

Techniques: In Vitro, Knockdown, Quantitative RT-PCR, Expressing, Western Blot, CCK-8 Assay, Colony Assay, Flow Cytometry, Two Tailed Test

Journal: iScience

Article Title: KIAA1429 promotes clear cell renal cell carcinoma progression by regulating MYC mRNA stability

doi: 10.1016/j.isci.2026.115198

Figure Lengend Snippet: KIAA1429 promoted ccRCC cell migration and invasion in vitro (A and B) The effect of KIAA1429 knockdown on the migration and invasion of Caki-1 and ACHN cells were detected using a cell wound-healing assay (A) and transwell assay (B). (C) Western blot analysis of vimentin, N-cadherin, and E-cadherin protein expression in Caki-1 and ACHN cells with KIAA1429 knockdown. Data are presented as the means ± SEM. ∗ p < 0.05 by Student’s two-tailed t test.

Article Snippet: KIAA1429 Rabbit Polyclonal antibody , Proteintech , Cat#24761-1-AP.

Techniques: Migration, In Vitro, Knockdown, Wound Healing Assay, Transwell Assay, Western Blot, Expressing, Two Tailed Test

Journal: iScience

Article Title: KIAA1429 promotes clear cell renal cell carcinoma progression by regulating MYC mRNA stability

doi: 10.1016/j.isci.2026.115198

Figure Lengend Snippet: KIAA1429 promoted tumorigenesis of ccRCC cells in vivo (A) Subcutaneous tumor model of ACHN cells with KIAA1429 knockdown. KIAA1429 knockdown led to decreased tumor volume. (B and C) The changes in volume and weight were measured at the indicated weeks after mice were transplanted with the relevant cells. (D) IHC of vimentin, Ki-67, and E-cadherin protein expression in tumors with KIAA1429 knockdown. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Article Snippet: KIAA1429 Rabbit Polyclonal antibody , Proteintech , Cat#24761-1-AP.

Techniques: In Vivo, Knockdown, Expressing, Two Tailed Test

Journal: iScience

Article Title: KIAA1429 promotes clear cell renal cell carcinoma progression by regulating MYC mRNA stability

doi: 10.1016/j.isci.2026.115198

Figure Lengend Snippet: MYC might be a potential target for regulation by KIAA1429 in ccRCC (A) KIAA1429 has a significant effect on most RNA-specific processes, cell cycle, cell death, apoptosis, and intracellular signaling pathways, as revealed by GO and KEGG analyses. (B) Heatmap illustrating the downregulated genes in ACHN cells after KIAA1429 knockdown. (C) The expression level of MYC in ccRCC, obtained from the TCGA database. (D) Correlation analysis between the expression of MYC and KIAA1429. Data are presented as the means ± SEM. ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Article Snippet: KIAA1429 Rabbit Polyclonal antibody , Proteintech , Cat#24761-1-AP.

Techniques: Protein-Protein interactions, Knockdown, Expressing, Two Tailed Test

Journal: iScience

Article Title: KIAA1429 promotes clear cell renal cell carcinoma progression by regulating MYC mRNA stability

doi: 10.1016/j.isci.2026.115198

Figure Lengend Snippet: KIAA1429 regulated the expression of MYC mRNA and its stability in an m6A-dependent manner (A and C) KIAA1429 knockdown inhibits the protein and mRNA expression of MYC in ccRCC cells. (B) KIAA1429 knockdown decreased the stability of MYC mRNA. (D) RIP assay showed that KIAA1429 interacts with MYC mRNA in Caki-1 and ACHN cells. (E) KIAA1429 knockdown reduced overall m6A levels in Caki-1 and ACHN cells. (F) KIAA1429 knockdown reduced m6A levels in MYC. (G) m6A methylation sites on MYC were predicted using the SRAMP database. (H) The pmirGLO-MYC-WT or pmirGLO-MYC-MUT luciferase reporter was transfected into KIAA1429-knockdown and control Caki-1 and ACHN cells, and relative luciferase activity was measured. Data are presented as the ratio of firefly to Renilla luciferase activity. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Article Snippet: KIAA1429 Rabbit Polyclonal antibody , Proteintech , Cat#24761-1-AP.

Techniques: Expressing, Knockdown, Methylation, Luciferase, Transfection, Control, Activity Assay, Two Tailed Test

Aberrant expression and prognostic ability of KIAA1429 in a variety of cancers. (a) Expression of KIAA1429 in various cancer tissues and normal tissues in GEPIA. (b) The expression of KIAA1429 in LUAD (red) is higher than that in normal lung tissue (gray) in GEPIA; T, Tumor; N, Normal. (c) High expression of KIAA1429 from GEPIA was significantly ( p < 0.001) related to the poor overall survival of LUAD patients. GEPIA, Gene Expression Profiling Interactive Analysis; LUAD, lung adenocarcinoma; KIAA1429 (VIRMA), vir‐Like m6A, methyltransferase associated; TCGA, The Cancer Genome Atlas.

Journal: Cancer Innovation

Article Title: Comprehensive analysis of the prognostic impact and immune implication of KIAA1429 in lung adenocarcinoma

doi: 10.1002/cai2.40

Figure Lengend Snippet: Aberrant expression and prognostic ability of KIAA1429 in a variety of cancers. (a) Expression of KIAA1429 in various cancer tissues and normal tissues in GEPIA. (b) The expression of KIAA1429 in LUAD (red) is higher than that in normal lung tissue (gray) in GEPIA; T, Tumor; N, Normal. (c) High expression of KIAA1429 from GEPIA was significantly ( p < 0.001) related to the poor overall survival of LUAD patients. GEPIA, Gene Expression Profiling Interactive Analysis; LUAD, lung adenocarcinoma; KIAA1429 (VIRMA), vir‐Like m6A, methyltransferase associated; TCGA, The Cancer Genome Atlas.

Article Snippet: Following blocking, rabbit anti‐KIAA1429 polyclonal antibody (1:100, HPA002037; Sigma‐Aldrich) was added to the TMAs and the samples were incubated overnight at 4°C.

Techniques: Expressing

Univariate and multivariate Cox logistic regression analysis of OS in TCGA cohorts

Journal: Cancer Innovation

Article Title: Comprehensive analysis of the prognostic impact and immune implication of KIAA1429 in lung adenocarcinoma

doi: 10.1002/cai2.40

Figure Lengend Snippet: Univariate and multivariate Cox logistic regression analysis of OS in TCGA cohorts

Article Snippet: Following blocking, rabbit anti‐KIAA1429 polyclonal antibody (1:100, HPA002037; Sigma‐Aldrich) was added to the TMAs and the samples were incubated overnight at 4°C.

Techniques: Expressing

Features of molecular mutation based on KIAA1429 expression in LUAD. Waterfall heatmaps based on annotation information of the top 20 mutant genes in the high (a) and low (b) KIAA1429 mRNA expression groups. (c) Association between gene mutation frequency and KIAA1429 mRNA expression level by chi‐square test. LUAD, lung adenocarcinoma; mRNA, messenger RNA. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Cancer Innovation

Article Title: Comprehensive analysis of the prognostic impact and immune implication of KIAA1429 in lung adenocarcinoma

doi: 10.1002/cai2.40

Figure Lengend Snippet: Features of molecular mutation based on KIAA1429 expression in LUAD. Waterfall heatmaps based on annotation information of the top 20 mutant genes in the high (a) and low (b) KIAA1429 mRNA expression groups. (c) Association between gene mutation frequency and KIAA1429 mRNA expression level by chi‐square test. LUAD, lung adenocarcinoma; mRNA, messenger RNA. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Following blocking, rabbit anti‐KIAA1429 polyclonal antibody (1:100, HPA002037; Sigma‐Aldrich) was added to the TMAs and the samples were incubated overnight at 4°C.

Techniques: Mutagenesis, Expressing

Representative immunohistochemical (IHC) staining of KIAA1429 protein in LUAD TMA. (a, b) H&E staining of LUAD tissue. (c, d) Negative immunohistochemical staining of KIAA1429 in LUAD. (e, f) Positive immunohistochemical staining of KIAA1429 in LUAD. The three pictures on the left were taken at low magnification and the pictures on the right were taken at high magnification. (g) High expression of KIAA1429 is significantly associated with poor overall survival (OS) of LUAD patients in the National Cancer Center of China (NCC) cohort. H&E, hematoxylin and eosin; LUAD, lung adenocarcinoma; TMA, tissue microarray.

Journal: Cancer Innovation

Article Title: Comprehensive analysis of the prognostic impact and immune implication of KIAA1429 in lung adenocarcinoma

doi: 10.1002/cai2.40

Figure Lengend Snippet: Representative immunohistochemical (IHC) staining of KIAA1429 protein in LUAD TMA. (a, b) H&E staining of LUAD tissue. (c, d) Negative immunohistochemical staining of KIAA1429 in LUAD. (e, f) Positive immunohistochemical staining of KIAA1429 in LUAD. The three pictures on the left were taken at low magnification and the pictures on the right were taken at high magnification. (g) High expression of KIAA1429 is significantly associated with poor overall survival (OS) of LUAD patients in the National Cancer Center of China (NCC) cohort. H&E, hematoxylin and eosin; LUAD, lung adenocarcinoma; TMA, tissue microarray.

Article Snippet: Following blocking, rabbit anti‐KIAA1429 polyclonal antibody (1:100, HPA002037; Sigma‐Aldrich) was added to the TMAs and the samples were incubated overnight at 4°C.

Techniques: Immunohistochemistry, Staining, Expressing, Microarray

Correlations between KIAA1429 expression and clinicopathological parameters of 415 patients with LUAD in NCC cohort 1

Journal: Cancer Innovation

Article Title: Comprehensive analysis of the prognostic impact and immune implication of KIAA1429 in lung adenocarcinoma

doi: 10.1002/cai2.40

Figure Lengend Snippet: Correlations between KIAA1429 expression and clinicopathological parameters of 415 patients with LUAD in NCC cohort 1

Article Snippet: Following blocking, rabbit anti‐KIAA1429 polyclonal antibody (1:100, HPA002037; Sigma‐Aldrich) was added to the TMAs and the samples were incubated overnight at 4°C.

Techniques: Expressing

Univariate and multivariable analysis of factors associated with overall survival in NCC cohort

Journal: Cancer Innovation

Article Title: Comprehensive analysis of the prognostic impact and immune implication of KIAA1429 in lung adenocarcinoma

doi: 10.1002/cai2.40

Figure Lengend Snippet: Univariate and multivariable analysis of factors associated with overall survival in NCC cohort

Article Snippet: Following blocking, rabbit anti‐KIAA1429 polyclonal antibody (1:100, HPA002037; Sigma‐Aldrich) was added to the TMAs and the samples were incubated overnight at 4°C.

Techniques: Expressing

KIAA1429 is associated with tumor‐infiltrating immune cells and predicts anti‐PD‐1 immunotherapy response in LUAD. (a) Correlation of KIAA1429 with major several immune cells and purity in LUAD. (b) Effects of genetic alterations associated with KIAA1429 expression on immune cell infiltration in patients. (c) Histogram of KIAA1429 mRNA expression in LUAD patients treated with anti‐PD‐1 immunotherapy. Blue and red represent stable disease (SD) and partial response (PR), respectively. (d) Scatter plot of KIAA1429 mRNA expression and response to anti‐PD‐1 immunotherapy. (e) Histogram of percentage of immunotherapy response rate and KIAA1429 mRNA expression level. (f) ROC curve of KIAA1429 in predicting response to LUAD immunotherapy. (g) The difference between KIAA1429 and PD‐L1 expression. (h) The difference between KIAA1429 expression and TMB. (i) The difference between KIAA1429 expression and IPS score. LUAD, lung adenocarcinoma; mRNA, messenger RNA; ROC, receiver operating characteristic.

Journal: Cancer Innovation

Article Title: Comprehensive analysis of the prognostic impact and immune implication of KIAA1429 in lung adenocarcinoma

doi: 10.1002/cai2.40

Figure Lengend Snippet: KIAA1429 is associated with tumor‐infiltrating immune cells and predicts anti‐PD‐1 immunotherapy response in LUAD. (a) Correlation of KIAA1429 with major several immune cells and purity in LUAD. (b) Effects of genetic alterations associated with KIAA1429 expression on immune cell infiltration in patients. (c) Histogram of KIAA1429 mRNA expression in LUAD patients treated with anti‐PD‐1 immunotherapy. Blue and red represent stable disease (SD) and partial response (PR), respectively. (d) Scatter plot of KIAA1429 mRNA expression and response to anti‐PD‐1 immunotherapy. (e) Histogram of percentage of immunotherapy response rate and KIAA1429 mRNA expression level. (f) ROC curve of KIAA1429 in predicting response to LUAD immunotherapy. (g) The difference between KIAA1429 and PD‐L1 expression. (h) The difference between KIAA1429 expression and TMB. (i) The difference between KIAA1429 expression and IPS score. LUAD, lung adenocarcinoma; mRNA, messenger RNA; ROC, receiver operating characteristic.

Article Snippet: Following blocking, rabbit anti‐KIAA1429 polyclonal antibody (1:100, HPA002037; Sigma‐Aldrich) was added to the TMAs and the samples were incubated overnight at 4°C.

Techniques: Expressing

KIAA1429‐related PPI network construction and biological function analysis. (a) PPI network constructed by 10 genes (CBLL1, METTL3, METTL14, RBM15, RBM15B, WTAP, ZC3H13, ZNF645, SNRNP200, and ALYREF genes) significantly related to KIAA1429. (b) GO terms including biological process (BP) and molecular function (MF) and KEGG pathways analysis. GSEA analysis of 188 lung cancer cell lines from CCLE yields the (c) neurotrophin signaling pathway, (d) cell cycle, (e) TGF‐BETA signaling pathway, and (f) basal transcription factors. CCLE, The Cancer Cell Line Encyclopedia; GO, Gene Ontology; GSEA, gene set enrichment analysis; KEGG, Kyoto Encyclopedia of Genes and Genomes; PPI, protein–protein interaction.

Journal: Cancer Innovation

Article Title: Comprehensive analysis of the prognostic impact and immune implication of KIAA1429 in lung adenocarcinoma

doi: 10.1002/cai2.40

Figure Lengend Snippet: KIAA1429‐related PPI network construction and biological function analysis. (a) PPI network constructed by 10 genes (CBLL1, METTL3, METTL14, RBM15, RBM15B, WTAP, ZC3H13, ZNF645, SNRNP200, and ALYREF genes) significantly related to KIAA1429. (b) GO terms including biological process (BP) and molecular function (MF) and KEGG pathways analysis. GSEA analysis of 188 lung cancer cell lines from CCLE yields the (c) neurotrophin signaling pathway, (d) cell cycle, (e) TGF‐BETA signaling pathway, and (f) basal transcription factors. CCLE, The Cancer Cell Line Encyclopedia; GO, Gene Ontology; GSEA, gene set enrichment analysis; KEGG, Kyoto Encyclopedia of Genes and Genomes; PPI, protein–protein interaction.

Article Snippet: Following blocking, rabbit anti‐KIAA1429 polyclonal antibody (1:100, HPA002037; Sigma‐Aldrich) was added to the TMAs and the samples were incubated overnight at 4°C.

Techniques: Construct

Information on antibodies used in this study.

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma

doi: 10.12659/MSM.919644

Figure Lengend Snippet: Information on antibodies used in this study.

Article Snippet: , KIAA1429 , 1: 200 , Rabbit polyclonal , Proteintech Group, China.

Techniques:

Review

kiaa1429 rabbit polyclonal antibody (Proteintech)

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1) Product Images from "KIAA1429 promotes clear cell renal cell carcinoma progression by regulating MYC mRNA stability"

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